商品详情：

Description:

CELLBANKER® 2 is a serum-free cell cryopreservation medium with unique complete formulation for broad spectrum of mammalian cell cultures. The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without potential risk of contamination serum.

Optimum for serum-free cultured cells and peptide/protein expressing cells cryopreservation.

Advantages: Safe and Save!

Safe: high cell viability, no risk of contamination serum derived pathogens, batch-to-batch stability.

Save: complete formulation, no further preparation, direct freezing at -80℃, no programmable freezer required, etc.

Protocol:

Freezing

For optimum results, cells for cryopreservation should be in log phase of growth. Similar or standard freezing protocols may be substituted.

1.Examine and make sure the cell culture free of contamination, in healthy situation and proper confluency, etc.

2.Perform a cell count to determine the viability of cells.

3.Gently pellet the cells centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4℃). Remove the supernatant using a aspirator.

4.Gently suspend the cells with CELLBANKER® cryopreservation medium (1 ml for 5x105 - 5x106 cells).

5.Dispense the cell suspension in 1ml aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.

6.Place the vials directly in a -80℃ for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.

IMPORTANT: Optimum protocol may change with the cell types.

Thawing

1.Remove the frozen cell from storage and quickly thaw in a 37 ℃ shaking waterbath.

2.Immediately dilute and gently mix each 1ml of cells with 10ml of complete cell culture medium.

3.Gently pellet the cells centrifugation (3-5 minutes at 1,000 - 2,000rpm, 4℃). Remove the supernatant aspirator.

4.Gently suspend the cells with appropriate volume of complete cell culture medium. And plate in a culture flask.

5.Continue the further culture procedures according to standard protocols.

Storage and Stability

Store at 2 to 8 or below -20 degrees Celsius. In unopened condition and under storage temperatures (2-8℃), the product will be stable for 3 years after the date of manufacture.